Does DOT Test For Synthetic Urine?

 The DOT drug test is carried out because it is believed that truck drivers offer safety-sensitive services and so they need to be in proper health and senses. But, does DOT test for synthetic urine? Let us find out.

Whenever an employee has to undertake a drug test, DOT informs the employee beforehand. This adds an unsaid advantage that the employee can easily make use of a fake pee kit and use synthetic urine to conveniently get through the drug test and simply save himself from the trouble and the consequences that might have been caused otherwise. 

Does DOT Test For Synthetic Urine?

DOT tests for urine samples but if you submit a sample of synthetic urine that is quality-oriented and has all the components that match the nutrients present in organic urine, the DOT drug test will not be able to find out that the specimen submitted by you is not real. DOT drug test only checks if the urine is biocide-free and at a proper temperature. If so, you will easily pass the drug. 

dot drug test

Will Synthetic Urine Pass a DOT Physical?

Yes, synthetic urine will pass a DOT physical if the fake pee sample that you have submitted during your DOT drug test has good quality and it does not contain biocide. 

Other factors to keep in check are-

The temperature of the Urine

DOT drug tests first check the urine for temperature. The temperature of the urine should range between 94 degrees Fahrenheit to 100 degrees Fahrenheit. If that temperature is maintained in the fake pee sample given by you for the drug test, you will likely get a negative drug test report. 

Color of the Urine

Quite understandably, organic urine has a pale yellow color, and whatever the synthetic urine that you are using, the color of the urine should be pale yellow or quite close to it. If not, the lab professionals can get suspicious and test your urine sample under a lot of drug test panels and the reports might become uncertain.


The DOT uses a Specimen validity testing method to detect substitution, adulteration, and dilution. So if you use low-quality synthetic urine, there is a high chance that the lab test will catch the synthetic urine.

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